This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:
"CRISPR-Cas9 genome editing has revolutionized biomedicine by enabling so-called genetic surgery on organisms ranging from single-cell microbes to sheep. Use of the technique, however, has been hampered by its low specificity, which can damage cells through unintended changes to DNA. New versions of the system have been engineered to avoid these off-target effects, but this has come at the cost of efficiency. Now, two separate teams have devised complementary ways to boost efficiency without sacrificing specificity, creating new promise for the field. The CRISPR-Cas9 system uses a protein called Cas9 and a guide RNA to make targeted breaks in DNA. When the guide RNA pairs with matching DNA, Cas9 cuts the DNA. The cut is then repaired by cellular machinery, and during this process, small pieces of DNA can be added or removed. One risk is that the system sometimes binds regions that aren’t exact matches, potentially causing unplanned changes..."
The rest of the transcript, along with a link to the research itself, is available on the resource itself.
- Subject:
- Genetics
- Life Science
- Material Type:
- Diagram/Illustration
- Reading
- Provider Set:
- Video Bytes
- Date Added:
- 09/20/2019
