Short Description:
A book created by students to learn about this unique microbe...
Word Count: 4024
(Note: This resource's metadata has been created automatically by reformatting and/or combining the information that the author initially provided as part of a bulk import process.)
How a cell can use a molecule's electrochemical gradient to power secondary active transport.
Common elemental building blocks of biological molecules: Carbon, Oxygen, Hydrogen, Nitrogen and Phosphorus.
This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:
"Insulin resistance and its progression to type 2 diabetes mellitus is an important public health concern. Both endoplasmic reticulum (ER) stress and reduced levels of the regulatory protein PGC-1α have been implicated in insulin resistance, but little is known about any interactions between them in this context. A recent study used cultured human skeletal muscle cells and mouse experiments to examine these potential interactions. In both cultured cells and mice, induced ER stress led to a decrease in PGC-1α and an increase in expression of ATF4, a transcription factor. To see if ATF4 was influencing PGC-1α expression, researchers increased ATF4 expression without ER stress, which also decreased PGC-1α expression, and reducing ATF4 before inducing ER stress blocked the drop in PGC-1α. ER stress activated mTOR, a major regulatory protein, and reduced levels of CRTC2, which is a transcription co-activator that increases PGC-1α transcription..."
The rest of the transcript, along with a link to the research itself, is available on the resource itself.
This project-based laboratory course provides students with in-depth experience in experimental molecular genetics, using modern methods of molecular biology and genetics to conduct original research. The course is geared towards students (including sophomores) who have a strong interest in a future career in biomedical research. This semester will focus on chemical genetics using Caenorhabditis elegans as a model system. Students will gain experience in research rationale and methods, as well as training in the planning, execution, and communication of experimental biology.
WARNING NOTICE
The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such safety procedures and measures. MIT shall have no responsibility, liability, or risk for the content or implementation of any of the material presented.
Legal Notice
Designed for students without previous experience in techniques of cellular and molecular biology, this class teaches basic experimental techniques in cellular and molecular neurobiology. Experimental approaches covered include tissue culture of neuronal cell lines, dissection and culture of brain cells, DNA manipulation, synaptic protein analysis, immunocytochemistry, and fluorescent microscopy.
This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:
"A new method for identifying post-translational modifications in proteins promises to cut biomedical researchers’ workload in half. Enabling multiple affinity enrichment procedures to be run in parallel, the one-pot method yields the same search results as traditional methods in less time and from less tissue. As proteomics researchers know well, identifying post-translational modifications in biological samples can be tedious. Enriching samples with target modifications, such as the attachment of acetyl , succinyl or methyl groups to amino acid residues, and matching experimental data with catalogued results involves numerous steps. And the work load is only getting bigger. With exploding interest in how multiple modifications are linked across the vast proteome , the amount of time and the amount of sample required for exploration are skyrocketing in proportion. But with the new one-pot enrichment method, that could soon change..."
The rest of the transcript, along with a link to the research itself, is available on the resource itself.
This course introduces the basic driving forces for electric current, fluid flow, and mass transport, plus their application to a variety of biological systems. Basic mathematical and engineering tools will be introduced, in the context of biology and physiology. Various electrokinetic phenomena are also considered as an example of coupled nature of chemical-electro-mechanical driving forces. Applications include transport in biological tissues and across membranes, manipulation of cells and biomolecules, and microfluidics.
Instructional video on the formation of catenane after replication of circular bacterial chromosome.
Although textbooks describe this process and show illustrations, it is difficult to grasp without seeing a live demonstration.
Created for Biology 41 General Genetics at Tufts University.
Since the discovery of the structure of the DNA double helix in 1953 by Watson and Crick, the information on detailed molecular structures of DNA and RNA, namely, the foundation of genetic material, has expanded rapidly. This discovery is the beginning of the “Big Bang” of molecular biology and biotechnology. In this seminar, students discuss, from a historical perspective and current developments, the importance of pursuing the detailed structural basis of genetic materials.
This problem challenges students to design experiments using techniques measuring gene expression (reverse transcriptase PCR, microarrays, in situ hybridization).
An integrated course stressing the principles of biology. Life processes are examined primarily at the molecular and cellular levels. Intended for students majoring in biology or for non-majors who wish to take advanced biology courses.
In this lab activity students isolate genomic DNA from their cheek cells on the inside of their mouths. Students then remove the DNA from those cheek cells. It shows the DNA is in every cell in the body and can be extracted easily. Students use their DNA necklace which they proudly wear around school the rest of the day.
Students are given a problem about a relatively new treatment for cancer, Gleevec, and asked to apply and synthesize what they have learned about cell signaling and the eukaryotic cell cycle to explain why this targeted treatment works to prevent cell division with fewer side effects than traditional chemotherapeutic agents.
Instructional video on how DNA binding proteins recognize their target sequences in DNA.
Although textbooks describe this process and show illustrations, it is difficult to grasp without seeing a live demonstration.
Created for Biology 41 General Genetics at Tufts University.
This activity is a lab investigation in which students design and conduct experiments using pineapple juice containing the enzyme bromelain and its affect on the substrate gelatin found in Jell-O. The focus of student driven investigations are on enzyme specificity, activity and the impact of environmental factors on enzyme functioning. Based on the original activities from School Improvement in Maryland; "Pineapple/Jell-O Lab," Access Excellence Activities Exchange; "Enzyme Labs Using Jell-O" by Anne McDonald and Michael O'Hare, and AP & Regents Biology; "Lab 8: Pineapple Enzymes and Jell-O Molds" by Kim B. Foglia.
In this video segment adapted from NOVA: Judgment Day: Intelligent Design on Trial, learn how modern genetics and molecular biology offer compelling support for evolution. The video features an interview with biologist Ken Miller.
Short Description:
This "textbook" is interactive, meaning that although each chapter has text, they also have interactive HTML5 content, such as quizzes, simulations, interactive videos, and images with clickable hotspots. Students receive instant feedback when they complete the interactive content, and therefore, can learn and check their understanding all in one place. The first unit introduces students to the nature of science, including scientific controversies, and information literacy, including how to analyze literature and identify stakeholders. Unit 2 is organismal biology, including carbon cycling and population growth, and unit 3 is molecular biology with a focus on gene expression.
Long Description:
This “textbook” is interactive, meaning that although each chapter has text, they also have interactive HTML5 content, such as quizzes, simulations, interactive videos, and images with clickable hotspots. Students receive instant feedback when they complete the interactive content, and therefore, can learn and check their understanding all in one place. I still consider this textbook to be fairly text-heavy and will continue to make it even more interactive content!
The image on the cover represents the creation of this book. I pulled most of the content from open resources, modified them, added questions, and now offer them for you to use!
I chose the content to align with two courses that I teach: environmental and organismal applications and biomedical applications. Unit 1 introduces students to science, which both courses use. Unit 2 covers content necessary for understanding conservation implications (the underlying theme of the course is de-extinction), and Unit 3 focuses on proteins so that students can understand the implications of modifying DNA (the underlying theme is CRISPR).
Please use this book as you see fit for your classes. I look forward to hearing how to make this book even more useful in the future!
Word Count: 27692
ISBN: 978-1-62610-106-7
(Note: This resource's metadata has been created automatically by reformatting and/or combining the information that the author initially provided as part of a bulk import process.)
Short Description:
This "textbook" is interactive, meaning that although each chapter has text, they also have interactive HTML5 content, such as quizzes, simulations, interactive videos, and images with clickable hotspots. Students receive instant feedback when they complete the interactive content, and therefore, can learn and check their understanding all in one place. The first unit introduces students to the nature of science, including scientific controversies, and information literacy, including how to analyze literature and identify stakeholders. Unit 2 is organismal biology, including carbon cycling and population growth, and unit 3 is molecular biology with a focus on gene expression.
Long Description:
This “textbook” is interactive, meaning that although each chapter has text, they also have interactive HTML5 content, such as quizzes, simulations, interactive videos, and images with clickable hotspots. Students receive instant feedback when they complete the interactive content, and therefore, can learn and check their understanding all in one place. I still consider this textbook to be fairly text-heavy and will continue to make it even more interactive content!
The image on the cover represents the creation of this book. I pulled most of the content from open resources, modified them, added questions, and now offer them for you to use!
I chose the content to align with two courses that I teach: environmental and organismal applications and biomedical applications. Unit 1 introduces students to science, which both courses use. Unit 2 covers content necessary for understanding conservation implications (the underlying theme of the course is de-extinction), and Unit 3 focuses on proteins so that students can understand the implications of modifying DNA (the underlying theme is CRISPR).
Please use this book as you see fit for your classes. I look forward to hearing how to make this book even more useful in the future!
Word Count: 34749
(Note: This resource's metadata has been created automatically by reformatting and/or combining the information that the author initially provided as part of a bulk import process.)
The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. Biological function at the molecular level is particularly emphasized and covers the structure and regulation of genes, as well as, the structure and synthesis of proteins, how these molecules are integrated into cells, and how these cells are integrated into multicellular systems and organisms. In addition, each version of the subject has its own distinctive material.
7.012 focuses on the exploration of current research in cell biology, immunology, neurobiology, genomics, and molecular medicine.
Acknowledgments
The study materials, problem sets, and quiz materials used during Fall 2004 for 7.012 include contributions from past instructors, teaching assistants, and other members of the MIT Biology Department affiliated with course #7.012. Since the following works have evolved over a period of many years, no single source can be attributed.