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Benzonase pre-digest successfully reduces DNA from dead bacteria and the host
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This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Next-generation sequencing (NGS) studies have led to dramatic improvements in our understanding of human microbiomes. However, this method is based on the presence of DNA and cannot distinguish between living and dead microbes on its own. Environments like our skin are hostile and have high microbe turnover, which leads to significant amounts of DNA from dead microbes, which can lead to inaccurate community estimations in NGS studies. To overcome this, researchers tested the feasibility of pre-treatment with Benzonase to digest unprotected DNA. They used both mock bacterial communities and skin microbiome samples with inactivated bacteria or bacteria-free DNA spiked-in. Benzonase (BDA) pre-treatment reduced the levels of DNA from dead bacteria in both mock and natural communities. It also reduced the amount of host DNA in samples with high human-to-microbial DNA ratios without obvious impact on the microbial profile..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

Subject:
Biology
Life Science
Material Type:
Diagram/Illustration
Reading
Provider:
Research Square
Provider Set:
Video Bytes
Date Added:
10/14/2021
Biochemistry Laboratory
Conditional Remix & Share Permitted
CC BY-NC-SA
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The course, which spans two thirds of a semester, provides students with a research-inspired laboratory experience that introduces standard biochemical techniques in the context of investigating a current and exciting research topic, acquired resistance to the cancer drug Gleevec. Techniques include protein expression, purification, and gel analysis, PCR, site-directed mutagenesis, kinase activity assays, and protein structure viewing.
This class is part of the new laboratory curriculum in the MIT Department of Chemistry. Undergraduate Research-Inspired Experimental Chemistry Alternatives (URIECA) introduces students to cutting edge research topics in a modular format.
Acknowledgments
Development of this course was funded through an HHMI Professors grant to Professor Catherine L. Drennan.

Subject:
Biology
Life Science
Material Type:
Full Course
Provider Set:
MIT OpenCourseWare
Author:
Taylor, Elizabeth
Date Added:
02/01/2009
L’organizzazione  e la funzione dell’apparato digerente
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Nell'attività di recupero progettata si ripropongono le FASI della digestione, i nutrienti e le necessità dell'organismo, l'anatomia dell'apparato digerente. La digestione meccanica e chimica in bocca en ello stomaco, il passaggio del chimo nell'intestino tenue.OBIETTIVIElencare le diverse FASI della digestione; individuare i nutrienti Indispensabili per Il corpo umano, identificando il ruolo svolto da ciascuno di essi, descrivere l'organizzazione e le funzioniI dei tessuti che  rivestono il tubo digerente.Distinguere la digestione meccanica dalla digestione chimica: descrivere le FASI della digestione nella bocca, nello stomaco, nell'intestino tenue, indicando le FUNZIONI delle sostanze secrete dal tubo digerente.TRAGUARDI FORMATIVIComprendere che il processo digestivo ha le FUNZIONI di elaborare gli alimenti trasformandoli in sostanze utilizzabili dalle nostre cellule.Saper m ettere in relazione I diversi organi che compongono l'apparato digerente con le rispettive FUNZIONI. Spiegare i processi fisici e  chimici implicati nelle FASI di trasformazione del cibo e dell'assorbimento dei nutrienti.Dalla bocca all'intestino 

Subject:
Anatomy/Physiology
Material Type:
Lesson Plan
Author:
Giuseppina polera
Date Added:
03/10/2017